In this blog, we will look at what to do once your gene editing experiment is finished and the different ways you will be able to characterize the successfully modified cell lines.
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CRISPR gene editing and siRNA gene silencing require the efficient delivery of the necessary reagents. This is easily achieved by optimizing delivery conditions. In this blog post we discuss techniques for optimization of a reverse transfection conditions and how to set up a 96 well plate to test 30 transfection conditions.
Learn how to use Dharmacon™ Edit-R™ CRISPR reagents to make functional knockout models in human iPSCs.
Discover Pin-point™, a first-generation base editing technology that we have licensed from Rutgers, a system we’ve since developed further to accelerate therapeutic development. Read the popular article now and see more of our recent advancements in the base editing space.
New advances in the use of base editing technology for treating sickle cell disease highlight the therapeutic promise of base editing in cell and gene therapy.
Guidelines for making the most out of your CRISPR experiment
Learn how precise control of Cas9 expression helps reveal the function of essential genes.
If you are wondering the best way to monitor the result of your gene editing experiment, we have reviewed the most commonly applied assays used.
Ready for gene editing? Get started with our All-in-one lentiviral system, an easy to use, single reagent for CRISPRko.
A proof-of-concept study where CRISPR-Cas9 reagents and guides to 3 genes were added to the same cell population and then a representative subset of clonal cell lines were analyzed for multiplexed gene editing efficiency.